UHPLC-MS/MS Determination of Seven Aminophenols in Hair Dyes

A method of UHPLC-MS/MS for simultaneous determination of seven aminophenols,including 2-amino-3-nitrophenol,4-amino-2-nitrophenol,2-amino-5-nitrophenol,2-amino-4-chlorophenol,3-diethylaminophenol,1,7-dihydroxynaphthalene and 2,3-dihydroxynaphthalene in hair dyes was proposed.The aminophenols in sample were extracted with methanol,and separated on Waters ACQUITY UPLCTM BEH C18 column and external standard method was used in the determination.The multi-reaction monitoring (MRM) mode was used for MS data collection.The values of detection limits (3S/N) for the 7 phenols found were less than 50.0 μg·L-1.Tests for recovery were carried out at three concentration levels of 10,20,50μg·g-1,giving values of recovery in the range from 68.8% to 112.5% and RSD (n=6) in the range from 1.58% to 12.61%.