HPLCMS/MS Determination of Na2EDTA in Food

HPLCMS/MS was applied to the determination of Na2EDTA in food. Na2EDTA in liquid samples (5 g) was extracted directly with 40 mL of water; while in solid or jam samples, was extracted by mixing and centrifuging with 15 mL of H2O and 20 mL of CHCl3, the supernatant was collected, and the lower phase was reextracted twice, the supernatants were collected and combined. The combined extract was then derivatized with FeCl3 solution by mixing ultrasonically for 10 min, and the mixture was made up to 50 mL. An aliquot of 5 mL of the derivatized solution was taken and purified by passing through MAX SPE column. The column was eluted with 6 mL of a mixture of HCOOHCH3OHH2O (10+50+40). The eluate was evaporated to dryness by N2blowing at 80 ℃, and the residue was dissolved with water. This solution was used for HPLCMS/MS analysis. Linearity range was found between 1.0-50.0 mg·L-1 for Na2EDTA. Detection limit (3S/N) found was 5 mg·kg-1. Four samples were analyzed by this method and test for recovery was made by standard addition method. Values of recovery obtained were in the range of 88.3%-96.0%, with RSD′s (n=6) all less than 10%.