HPLC Determination of 3 Components of Sodium Nitrophenolate Compound

HPLC was applied to the determinations of 3 components of a phytohormone called sodium nitrophenolate compound, including sodium 5- nitroguaiacol, sodium o-nitrophenol and sodium p-nitrophenol. Hypersil BDS C18 (200 mm×4.6 mm, 5 μm) column was used as stationary phase, and a mixed solution of methanol and water (mixed in the ratio of 55 to 45) was used as mobile phase. The 3 components from the column were determined by HPLC with diode array detector at the wavelength of 235 nm. Linear relationships between the values of peak area and mass concentration of the 3 components were found same in the range of 5-300 mg·L-1. The values of recovery found by standard addition method were in the range of 99.5%-101.7%. Precision was tested by addition of 50 mg·L-1 of mixed standard of the 3 components, values of RSD′s (n=6) found for the 3 components were 0.46%, 0.37% and 0.53% respectively.