Fluorospectrophotometric Determination of Trace Amount of Bromate in Drinking Water by the Fluorescence Quenching of Acridine Orange

Based on the fluorescence quenching effect produced by oxidation of acridine orange with BrO3- in the presence of KBr in a HCl medium, a method for the determination of trace amount of BrO3- in drinking water was proposed. Linear relationship between magnitude of decrease in fluorescence intensity (ΔF) and mass concentration of BrO3- was kept in the range of 10.0-560.0 μg·L-1, when measured at (λex) 493 nm and (λem) 528 nm. Value of detection limit (3S/N) found was 3.03 μg·L-1. The proposed method was applied to the determination of bromate in purified water and barreled water, giving values of recovery by standard addition method in the range of 94.0%-107%.