Capillary Electrophoretic Determination of 3 Nucleotides in G-P Health-care Capsule

A method for the simultaneous separation and determination of 3 nucleotides,i.e.adenosine 5′-monophosphate (AMP),cytidine 5′-monophosphate (CMP) and guanosine 5′-monophosphate (GMP),in the health-care capsule containing glucoside and peptide (abbreviated as G-P capsule) by capillary electrophoresis was proposed.A mixture of solutions of 15 mmol·L-1 sodium borate,35 mmol·L-1 sodium carbonate and 60 mmol·L-1 hydroxypropyl-β-cyclodextrin was used as background electrolyte,and moving voltage of 30 kV was applied.UV-detection at the wavelength of 254 nm was adopted.Linearity ranges between 5.0-80.0 mg·L-1 (for AMP) and 8.0-80.0 mg·L-1 (for CMP and GMP) were obtained with detection limits (3S/N) of 2.0,3.0 and 3.0 mg·L-1 respectively.Recovery and precision of the method were tested by standard addition method,the results of recovery found were in the range of 92.1%-102.7% and RSD′s (n=5) were less than or equal to 5.0%.