Capillary Electrophoresis-Electrochemiluminescence Determination of Tramadol in Preparations of Tramadol Hydrochloride and Blood Plasma

By using capillary electrophoresis in combination with the end-column electrochemiluminescence detection (CE-ECL) which is based on the chemiluminescence reaction between tris-(2,2′-bipyridine) ruthenium Ru(bpy)2+3 and dissolved oxygen in PBS of pH 9 at the Pt-disc working electrode by the tert amino group present in the molecule of tramadol,a novel method for determination of tramadol in its preparations and in blood plasma was proposed.Optimum conditions of CE and ECL were studied and described in detail.Linearity ranges between 1.0×10-8-7.0×10-6mol·L-1 (for determination of tramadol in preparations) and 2.0×10-7-6.0×10-6mol·L-1 (for determination of tramadol in blood plasma) were obtained with detection limits (3S/N) of 7.0×10-9mol·L-1 and 1.0×10-7mol·L-1 respectively.Recovery and precision of the method were tested by standard addition method at 3 different concentration levels,the results of average recovery found were 98.9% (for preparations) and 89.9% (for blood plasm).