HPLC DETERMINATION OF GLYOXALIC ACID IN PRESENCE OF GLYCOLIC ACID AND OXALIC ACID

A simple and rapid reversed-phase high performance liquid chromatographic method was applied to the determination of the content of glyoxalic acid in the presence of glycolic acid and oxalic acid.Hypersil ODS C18 column,a mobile phase of acetonitrile-water mixed solution and UV detection at 210 nm were employed in the determination.The effects of pH and composition of the mobile phase on the sensitivity and separation of the method were studied.Linearity between the peak area and concentration of glyoxalic acid (g·L-1) was tested and the following regression equation was obtained Y=1.934×103X+0.067×103 (r=0.999 9).The detection limit (2S/N) was found to be 5×10-7g·L-1.Test for precision at the same concentration for 7 determinations gave a value of RSD of 0.4%.Recoveries obtained by the standard addition method were in the range of 98.1%-100.4%.