HPLC Determination of Benzo［a］pyrene in Grease after Purification with Gel Permeation Chromatography

A method for determination of benzo［a］pyrene (BaP) in grease by HPLC was proposed. The samples was extracted with mixed solvent of n-hexane and ethyl acetate (1+1). The extract obtained was purified to remove the macromolecular impurities by gel permeation chromatography. The eluates were taken for separation by HPLC and the content of BaP was determined by fluorescence detector. Linear relationship between values of peak area and mass concentration of BaP was kept in the range of 5.00-200 μg·L-1, with detection limits (3S/N) of 3 μg·L-1. Tests for recovery were made by addition of standard solution at 3 different concentration levels, values of recovery found were in the range of 85.6%-97.8% with RSD (n=6) less than 3.0%.