GC-MS Determination of 2-Alkylcyclobutanones in Irradiated Fish Oil with Solid Phase Extraction

A method of GC-MS for the determination of 2-alkylcyclobutanones,i.e.2-dodecylcyclobutanone and 2-tetradecylcyclobutanone in irradiated fish oil with solid phase extraction was proposed.The sample (0.300 g) of irradiated fish oil was dissolved with n-hexane,and cleaned up with a ProElut Silica solid phase extraction column.The HP-5MS chromatographic column was used for GC separation and the selected ion monitoring mode was adopted in MS determination.Linear relationships between values of peak area and mass concentration of the two 2-alkylcyclobutanones were kept in the same range of在0.01-1.0 mg·L-1,with lower limits of determination (10S/N) of 10 μg·kg-1.Values of recovery found by standard addition method were in the range of 91.2%-96.0%,with RSD′s (n=6) in the range of 3.7%-5.9%.