FI-Inhibitory Chemiluminescence Determination of Carbofuran

Based on the inhibitory effect of carbofuran on the CL-reaction system of luminol-H2O2-sodium copper chlorophyll in an alkaline medium, a method for the determination of carbofuran by FI-inhibitory chemiluminescence (CL) was proposed. It was found that a stable complex was formed by the reaction of sodium copper chlorophyll with carbofuran in the mole ratio of 1 to 1, the process of quenching of fluorescence of carbofuran was proved to be a process of static quenching. Equilibrium constant (K0) and combination distance (r) the complex were determined and values obtained were 3.41×105L·mol-1 and 0.39 nm respectively. Linear relationship was found between values of CL intensity and mass concentration of carbofuran in the range of 0.08 to 2.0 mg·L-1, and detection limit (3σ/k) of the method found was 0.03 mg·L-1. The proposed method was used in analysis of carbofuran in secticide samples, giving values of average recovery of 101.5%.