RP-HPLC Determination of Aripiprazole and its Metabolite in Blood Serum

A method of RP-HPLC for determination of aripiprazole and its metabolite,dehydro-aripiprazole was proposed.Sample of blood serum was extracted with ethyl ether and the eluate obtained was evaporated to dryness by N2-blowing at 35 ℃.The residue was dissolved with the mobile phase mixture and separated by passing through Zorbax-C18 column,and the eluate from the column was used for HPLC analysis with UV detector at the wavelength of 254 nm.Linearity ranges of aripiprazole and dehydro-aripiprazole were kept from 4.0 to 2 000 μg·L-1 and 4.0 to 1 500 μg·L-1 respectively,with same lower limits of determination (10S/N) of 4.0 μg·L-1.Values of relative recovery of aripiprazole and dehydro-aripiprazole in the range of 100.3%-102.7% and 93.7%-103.9% were found respectively.Values of intraday and interday relative standard deviation (n=5) were all less than 7%.