Determination of Ferulic Acid in “MAI-DANG-RU-TONG” Granules by High Performance Capillary Electrophoresis

Ferulic acid in a ready-made Chinese herbal medicine,called “MAI-DANG-RU-TONG” (in granules) was determined by high performance capillary zone electrophoresis (HPCZE).Electrophoretic separation was performed on an uncoated fused-silica capillary at 18 ℃.A 12.5 mmol·L-1 borax buffer solution of pH 8.20 was used as moving buffer.Sample introduction was conducted hydrodynamically at 6 kPa for 3 s,with detection wavelength at 200 nm.Linearity was kept in the concentration range of 1-52 mg·L-1 of ferulic acid with correlation coefficient of 0.999 9.Average recovery of 98.77% and RSD value of 1.9% were obtained.Values of intra-day and inter-day RSD were found to be 1.9% and 1.6% respectively.