FI-Chemiluminescence Determination of Diosmetin

Based on the sensitization effect of diosmetin on the CL-reaction system of luminol-K3Fe(CN)6 in NaOH medium, a method for the determination of diosmetin by FI-chemiluminescence (CL) was proposed. Under the optimum conditions, linear relationship of values of CL intensity and mass concentration of diosmetin was obtained in the range of 5.0×10-6-5.0×10-4g·L-1, with detection limit (3σ) of 3.7×10-6g·L-1. The proposed method was applied to the analysis of simulated samples, giving values of recovery by standard addition method in the range of 89.6%-98.9% and values of RSD′s (n=5) in the range of 0.1%-3.2%.