Fluorescence Resonance Energy Transfer Probe Based on Nano-Ag-EY-DNA System

The resonance energy transfer between nano-Ag and eosin Y (EY)-DNA was studied by UV-Vis spectrophotometry, fluorospectrophotometry and electrophoresis with EY-DNA as fluorescence probe. The phenomenon of interaction between nano-Ag and EY-DNA was explained by the theory of fluorescence resonance energy transfer (FRET). It was found that the reaction of EY with DNA was due to inserting action, and the reaction between nano-Ag and DNA was due to electrostatic action. The structure of DNA was changed after binding with EY and nano-Ag.