HPLC Simultaneous Determination 5 Kinds of Additives in Pickles

HPLC was applied to the determination of 5 kinds of additives, including acesulfame potassium, saccharin sodium, benzoic acid, sorbic acid and dehydroaectic acid in pickles. The sample was extracted ultrasonically with 0.02 mol·L-1 NaOH solution and precipitated with Pb(CH3COO)2-Na2SO4 . After filtering on 0.45 μm filtering membrane, the filtrate was used for HPLC analysis. The Agilent C18 chromatographic column (250 mm×4.6 mm, 5 μm)was used for separation and a mixture of methanol and 0.02 mol·L-1 KH2PO4 solution (25+75) was used as the mobile phase. Diode array detector at the wavelengths of 225 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 5 additives were obtained in the range of 5-100 mg·L-1, with detection limit (2S/N) in the range of 1-2 mg·L-1. Values of recovery of the method found were in the range of 85.6%-105.8%, with RSD′s (n=6) less than 5.0%.