GC-MS Determination of Vanillin and Ethyl Vanillin in Tobacco Flavor

GC-MS was applied to the determination of vanillin and ethyl vanillin in tobacco flavor.The ratio of the sample of tobacco flavor and extractant was 1 g∶100 mL,and the sample was extracted with 500 W ultrasonic wave for 10 minutes.The DB-WAXETR capillary column was used for GC separation and the selected ion monitoring mode was adopted in MS determination.Phenylethyl propionate was used as internal standard.Linearity ranges of vanillin and ethyl vanillin were found in the same range of 0.5-200 mg·L-1 with detection limits (3S/N) of 0.03,0.02 mg·kg-1 respectively.Values of recovery found by standard addition method were in the range of 91.4%-109%.Values of within-day RSD′s (n=5) were in the range of 0.43%-2.2% and values of inter-day RSD′s (n=5) were in the range of 0.67%-2.6%.