Determination of Diphenylarsinic Acid in Pteris Vittata L. by HPLC

HPLC was applied for the determination of diphenylarsinic acid (DPAA) in Pteris vittata L.. The sample was extracted by oscillation in Na2HPO4 solution of 0.02 mol·L-1 for 4 h. After centrifugation,the supernatant was separated on a Shimadzu VP-ODS reserved-phase C18 column with acetonitrile-KH2PO4 solution of 0.02 mol·L-1 as the mobile phase for gradient elution. UV-detection was made at 220 nm. The linear relationship was found between the peak area and the mass concentration of DPAA was in the range of 0.20-2.0 mg·L-1,with detection limit of 2.8 mg·kg-1. Recovery rates measured by standard addition methods were in the range of 98.5%-98.8%,with RSD (n=5) in the range of 0.97%-4.6%. The method was used to determine DPAA in Pteris vittata L.