HG-ICP-AES Determination of Selenium in Blood Sample

Sample of blood was digested with mixed acid of HClO4-HNO3 (1+4), and the content of Se in the solution obtained was determined by HG-ICP-AES with addition of HCl as pre-reductant. In a 6 mol·L-1 HCl medium, hydrides of Se was generated by reacting with 5 g·L-1 NaBH4 solution (dissolved in 20 g·L-1 NaOH solution). Value of detection limits (3s/k) found was 1.8 μg·L-1. The proposed method was used in the determination of Se in 2 blood samples, giving values of recovery in the range of 94.9%-107% with RSD (n=10) less than 3%.