Separation and Determination of Amino Acids in Pharmaceutical Waste Liquor

Waste liquor from a vitamin B12 pharmaceutical factory was treated with lead acetate to precipitate protein and other macro-molecular substances and separated by centrifuging. Amino acids in the supernatant liquid were separated by passing through a column packed with strong-acidic cation exchange resin (26 cm×2.5 cm). Amino acids on the resin were eluted with 1 mol·L-1 ammonia water. Amino acids in the eluate were then analyzed both qualitatively and quantitatively by GC/MS and FT-infrared spectrometry.