High Performance Capillary Electrophoretic Determination of Residual Amounts of Penicillin Drugs in Chicken

Chicken samples were pretreated by the following 4 methods separately: ① NaH2PO4-extraction (SE), ② NaH2PO4-solid phase extraction (SE-SPE), ③ matrix solid-phase dispersion extraction (MSPDE)and ④ matrix solid-phase dispersion extraction-SPE (MSPDE-SPE). The contents of penicillin, ampicillin and amoxicillin in the sample solutions were determined by high performance capillary electrophoresis (HPCE). As shown by the results obtained by recovery tests and chromatographic separation, the pretreatment method ② was more suitable to be used for simultaneous determination of the 3 penicillin drugs. Tests for recovery and precision of the proposed method were tested, values of recovery and RSD′s (n=5) found were in the ranges of 73.1%-95.9% and 2.9%-9.8% respectively.