Simultaneous HPLC Determination of 4 Contraband Yellow Industrial Dyes in Yellow Croaker

HPLC was applied to the determination of 4 contraband yellow industrial dyes, i.e. auramine O, orange G, basic orange Ⅱ and metanil yellow, in yellow croaker. Kromasil C18 column was used as stationary phase for separation, and mixtures of methanol, water and 20 mmol·L-1 ammonium acetate solution in different ratios were used as mobile phase in gradient elution. UV-detection at 430 nm was adopted in the determination. Linearity ranges for the 4 above mentioned dyes were found between 0.05-2.0 mg·L-1, 1.0-15.0 mg·L-1, 0.1-5.0 mg·L-1 and 0.4-15.0 mg·L-1 respectively. Values of detection limit (3S/N) of the dyes were found in the range of 0.002 5-0.05 μg·kg-1. On the base of a yellow croaker sample, test for recovery was made by standard addition method, values of recovery found were in the range of 96.4%-98.1%, with RSD′s (n=6) in the range of 1.3%-2.5%.