GC-MS Determination of Restricted Ingredients in Beeswax Absolute

A method for determination of restricted ingredients in beeswax absolute by GC-MS was proposed. The sample was dissolved with mixed solvent of CH2Cl2-CHCl3 (1+1), separated on two chromatographic columns with different polarity, and determined by MS under select ion monitoring (SIM) mode. Ninety-six components were identified, among which the coumarin and mossoia lactone were included to restricted ingredients. Linearity ranges between values of peak area and mass concentration of 7 restricted ingredients, including benzoic alcohol, cinnamic alcohol, coumarin, mossoia lactone, benzyl benzoate, benzyl salicylate and benzyl cinnamate, were obtained in the same range of 0.08-80 mg·L-1, with detection limit (3S/N) in the range of 0.01-0.23 mg·kg-1. Values of recovery found by standard addition method were in the range of 86%-101%, with RSD′s (n=6) less than 4.0%.