GC-MS Determination of 11 Anabolic Steroids in Human Urine with Solid-Phase Extraction

Hyphenated GC-MS was applied to the simultaneous determination of 11 anabolic steroids in human urine. Urine sample was treated with C18 solid phase extraction (SPE) column to purify and concentrate the analytes. The eluate from the SPE column was evaporated to dryness and enzymehydrolyzed in PBS of ca. pH 7,extracted with tert. butyl methyl ether,and dried by blowing with nitrogen. To improve sensitivity of determination,prederivatization of the sample solution with silane was performed. GC-MS determination was carried out using methyl testosterone as internal standard and with the SIM mode. Detection limits (S/N=3) obtained were in the range of 0.1-2.0 μg·L-1. Tests for recovery and precision were made on the base of substantial samples,and values of average recovery found were in the range of 70.5%-121.0%,and values of RSD′s (n=5) were in the range from 1.1% to 13.9%.