Simultaneous LC Determination of Guanidine Hydrochloride and Chloridion in Bio-buffer with Charged Aerosol Detector

LC was applied to the simultaneous determinations of guanidine hydrochloride and chloridion in bio-buffer. The sample were separated on Acclaim Trinity P1 column (3.0 mm×100 mm, 3 μm) with the mobile phase containing, acetonitrile and 0.020 mol·L-1 NH4OAc solution, mixed in the ratio of 30 to 70 (by volume) and adjusted to pH 5.0 with acetic acid, and the contents of guanidine hydrochloride and chloridion were determined by charged aerosol detector with atomizing temperature of 25 ℃ and sampling frequency of 5 Hz. Linear relationships between peak area and mass concentration for guanidine hydrochloride and chloridion were obtained in the same range of 0.20-5.00 g·L-1, and with their detection limits (3S/N) of 0.005 and 0.001 g·L-1 respectively. Values of average recovery found by standard addition method were 96.0% for guanidine hydrochloride and 92.5% for chloridion, with values of RSD′s (n=7) less than 1.0%.