HPLC-MS/MS Determination of Residual Amounts of Prochloraz and 2,4,6-Trichlorophenol in Banana with Solid Phase Extraction

A method of HPLC-MS/MS for determination of residual amounts of prochloraz and 2,4,6-trichlorophenol in banana with solid phase extraction was proposed. The banana sample was extracted with a mixture of acetic acid and acetonitrile (2+98) and purified by matrix dispersion solid phase extraction. The purified sample solution was separated by using Agilent Eclipse AAA column as stationary phase, and mixtures of acetonitrile and water in different ratios as mobile phase in gradient elution. ESI+, ESI- and multi-reaction monitoring were adopted in MS/MS. Linearity range was found between 0.05-100 mg·L-1 for prochloraz, and between 2.5-200 mg·L-1 for 2,4,6-trichlorophenol, with detection limits (3S/N) of 0.02 μg·kg-1 for prochloraz and 1.5 μg·kg-1 for 2,4,6-trichlorophenol. Using blank samples as matrixes, test for recovery was made by standard addition method, giving recovery of prochloraz and 2,4,6-trichlorophenol in the ranges of 90.3%-106% and 85.5%-101% respectively, with RSD′s (n=6) in the ranges of 3.3%-8.5% and 3.6%-9.6% respectively.