SPE-HPLC Determination of Trace Amounts of Metsulfuron-Methyl in Water

High performance liqiud chromatography with solid phase extraction was applied to the determination of residual amount of metsulfuron-methyl in water. The sample was enriched and purified by Oasis MCX SPE column. The eluate obtained was separated on Extend C18 column (250 mm×4.6 mm,5 μm),using a mixture of 0.2 mol·L-1 formic acid,methanol and acetonitrile mixed in the ratio of 60 to 20 to 20 (by volume) as mobile phase. DAD-detection at 225 nm was used in the determination. Linear relationship between values of peak area and mass concentration of metsulfuron-methyl was kept in the range of 0.005-5.0 mg·L-1. Lower limit of determination (10S/N) of the method was found to be 0.005 mg·L-1. On the base of water sample,test for recovery were made by standard addition method,values of recovery found were in the range of 94.0%-102.0%,and values of RSD′s (n=6)were less than 6.5%.