Resolution of Enantiomers of Homoarginine by HPLC Using Mobile Phase Added with Chiral Additive

The complex of L-phenylalanine-Cu(Ⅱ) was used as chiral additive in the resolution of enantiomers of homoarginine (Harg.) by HPLC. The Waters Atlantis C18 column was used as the stationary phase, and a mixture of CH3OH and water (mixed in the ratio of 20+80) containing 0.71 mmol·L-1 L-phenylalanine and 0.351 mmol·L-1 CuSO4 (pH 2.8) was used as mobile phase, with a flow-rate of 0.5 mL·min-1. UV-detection at the wavelength of 200 nm was adopted for the determination, and volume of sample introduced was 20 μL. It was shown that enantiomers of Harg was successfully separated by the proposed method and value of separation factor achieved to 1.8. Linear relationships between values of peak area and mass concentration of D-Harg and L-Harg were obtained in the ranges of 2.00-72.05 mg·L-1 and 3.00-60.23 mg·L-1; values of detection limit (3S/N) found were 1.0 mg·L-1 and 1.5 mg·L-1 respectively.