Rapid Determination of Zearalenone in Wheat

HPLC with fluorescence emission intensity detection was used in the determination of zearalenone in wheat.Zearalenone was separated from the sample by extraction with a mixed solvent of acetonitrile and water (mixed in the vol.ratio of 84 to 16),and the extract was purified by passing through the multifunctional column.Chromatographic separation was carried out on the C18 column (4.6 mm×250 mm,5 μm) and eluted with a mixture containing water,acetonitrile and methanol (mixed in the vol.ratio of 46 to 46 to 8),flowing at a rate of 1.0 mL·min-1.Linear relationship between values of peak area and concentration of zearalenone was obtained in the range of 20.0 to 5 000.0 μg·L-1.Tests for recovery and precision were made at the concentration levels of 50.0,100.0 and 1 000.0 ng·g-1,and values of average recovery 74.6%,75.5%,73.5%,and values of RSD′s (n=8) calculated were in the range of 9.7%-11.5%.Lower limit of determination (10S/N) of the method found was 50.0 ng·g-1.