Determination of Sebacic Acid and Sebaconitrile by HPLC

A method of HPLC determination of sebacic acid and sebaconitrile using a stainless steel C18 column (25 cm×4.6 mm,5 μm) and differential refractive index detector was proposed.Adipic acid and hexanedinitrile were selected as internal standards for determination of sebacic acid and sebaconitrile respectively.Eluants of mixture of methanol and dil.HOAc (1+99),mixed in the ratio of 50 to 50 (by volume) and in the ratio of 40 to 60 (by volume) were used as mobile phase in the elution of sebacic acid and sebaconitrile respectively.Linear relationships between values of peak area ratio (or peak height ratio) and concentration of the analytes were obtained in the range of 100-900 mg·L-1 (for sebacic acid) and in the range of 0.227-8.17 g·L-1 (for sebaconitrile).