Capillary Column-Gas Chromatographic Determination of Monosaccharides with Pre-derivatization

A comparative study on the GC determination of monosaccharides with capillary chromatographic column and pre-derivatization was reported.Among the 3 commonly used methods for derivatization,the method of transforming the monosaccharides to aldononitrile acetate in the presence of NH2OH·HCl (pyridine solution) and acetic anhydride,was chosen.It was also shown that the capillary chromatographic columns of OV-1701 and FFAP were proved to be most efficient in separation and giving more symmetrical peak shape as compared with the other 3 columns tested.Inositol was used as internal standard in the determinations and 7 monosaccharides,i.e.,L-rhamnose,D-fucose,D-arabinose,D-xylose,D-mannose,D-glucose and D-galactose,were determined by the method.Linear relationships between values of analytical signals and concentration of the 7 monosaccharides were obtained with their linear regression equations given.Values of detection limit (3S/N) obtained were all less than 14 μg.Standards of the 7 monosaccharides were added to a known sample and test for recovery was done,giving results in the range of 92.5%-102.5%.Results of RSD′s (n=5) found by the precision test were all less than 2.5%.