HPLC Simultaneous Determination of Glucosamine Hydrochloride and Glucosaminic Acid in Catalytic Reaction Solution

The catalytic reaction solution used for preparation of glucosaminic acid was mixed with NaNO2 as derivatizing reagent, and derivatized for 5 min at 25 ℃. The solution containing derivatives of glucosamine hydrochloride and glucosaminic acid was used for HPLC analysis, using Aminex HPX-87H ion replusive column as stationary phase and 5 mmol·L-1 H2SO4 solution as mobile phase in isocratic elution. Differential refractive index detector was adopted in the determination. Linear relationships between values of peak area and concentration of the 2 compounds were obtained in the same range within 50 mmol·L-1, with detection limits (3S/N) 0.014 mmol·L-1 (for glucosamine hydrochloride) and 0.034 mmol·L-1 (for glucosaminic acid). The proposed method was applied to the determination of glucosamine hydrochloride and glucosaminic acid in catalytic reaction solution, giving values of recovery in the ranges of 99.3%-100% and 99.2%-102% with RSD′s less than 2.5%.