Determination of Residual Amounts of Tylosin and Sulfonamides in Meat by Surface Plasma Resonance Technology

Method for determination of residual amounts of tylosin and sulfonamides in meat by application of the surface plasma resonance technology was proposed. The sensor chip of CM5 was used as the resonance chip; a mixture of acetonitrile and 0.15 mol·L-1 NaOH solution (9+1) was used as the regeneration solution; and HBS-EP was used as the buffer solution with its flow-rate of 60 μL·min-1. It was found that the values of relative intensity of resonance produced on the surface of the sensor chip CM5 kept linear relationship with mass concentration of tylosin and sulfonamides in the ranges of 1-32 μg·L-1 and 0.5-50 μg·L-1 respectively, with values of detection limits (3S/N) of 0.27 μg·kg-1 (for tylosin) and 4.5 μg·kg-1 (for sulfonamides). Recovery was tested, giving values of recovery in the ranges of 80.0%-98.8% (for tylosin) and 77.3%-98.4% (for sulfonamides). RSD′s (n=6) found were 4.8% and 2.0% respectively.