RP-HPLC Determination of L-Carnitine in Feed and Feed Additives

RP-HPLC was applied to the determination of L-carnitine in feed additive and feed.The methods of sample extraction,and conditions of separation by liquid chromatography were tested and optimized.The Agela ASB VS851505-0 C8 column (150 mm×4.6 mm,5 μm) was used as stationary phase,and a mixed solution of methanol and phosphate buffer solution (mixed in the ratio of 5 to 95 by volume) was used as mobile phase.UV-detection at 210 nm was adopted in the determination.Linear relationship between the values of peak area and mass concentration of L-carnitine was obtained in the range of 0.025-4.0 mg·L-1.Values of recovery found by standard addition method were in range of 96.0%-101.2%,and values of RSD (n=5) were less than 3.0%.