UHPLC Determination of Metolcarb, Isoprocarb, Baycarb and Diethofencarb in Water with Solid Phase Extraction

UHPLC was applied to the determination of metolcarb, isoprocarb, baycarb and diethofencarb in water with solid phase extraction. The water sample (preadjusted to pH 4-5) was purified by passing through Oasis HLB-solid phase extraction column and eluted with methanol. Eclipse XDB C18 chromatographic column was used as stationary phase at column temperature of 30 ℃, and a mixture of methanol and water (70+30) was used as mobile phase. UV-detection at 284 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 4 pesticides were kept in definite ranges, with detection limits(3S/N )in the range of 0.049-1.6 μg·L-1.Tests for recovery were made at 3 concentration levels, giving values of recovery in the range of 92.0%-106 %with RSD′s (n=7)in the range of 1.2%-4.9%.