Componental Analysis of Bitter Almond for Fatty Acid Contents by GC-MS

Based on the database of standard chromatograms and mass spectra of GC-MS and on the regularities of organic mass spectrometry,the fragmentation patterns of methyl esters of straight chain saturated fatty acids,single unsaturated fatty acids and double unsaturated fatty acids were analyzed and concluded in detail.The number of carbon atoms and double bonds was determined on the basis of characteristic ions.The bitter almond sample was pre-derivatized by treating ultrasonically with the methyl esterifying agent,H2SO4-CH3OH solution and n-hexane,and components of fatty acids were separated on the PE-5MS capillary column,identified and determined by GC-MS.The method of peak area-normalization was adopted in the determination.Totally,86 compounds were separated,among which 32 compounds were determined with their contents (relative %) amounting to 95.8% of the total.