LC-MS/MS Determination of Oxidation Product Aniline in the Fenton Degradation of Azo Dye Orange G

LC-MS/MS was applied to the determination of oxidation product aniline in Fenton degradation of azo dye orange G. The Fe2+ in Fenton′s reaction solution was removed by OnGuard II H solid phase extraction column. After the extraction, the separation was performed on a XR-ODS II chromatographic column. Detection was carried out using a positive electrospray ionization interface in multi-reaction monitoring mode. Linearity range of aniline found was 2.0-100.0 μg·L-1 with detection limit (3S/N) of 0.6 μg·L-1. Values of recovery found by standard addition method were in the range of 94.0%-98.4%, with values of intra-day RSD′s and inter-day RSD′s in the ranges of 2.8%-6.4% and 5.2%-8.6% respectively.