UHPLC-MS/MS Determination of Dexamethasone Betamethasone and Beclomethasone in Pork

A method of determination of residual amounts of dexamethasone,betamethasone and beclomethasone in pork by hyphenation of UHPLC with MS/MS (in tandem) was proposed.The temdem quadripole mass spectrometer in hyphenation with ultra high performance liquid chromatography with ionization source of ESI+ and detection mode of MRM was used in the analysis.The C18 column was used as stationary phase,and mixtures of dil.formic acid (1+999) and acetonitrile in different proportions were used as mobile phase in the gradient elution of chromatographic separation.Linear relationships between values of analytical signal and mass concentration of the 3 drugs were obtained in the range from 1.0 to 100.0 μg·L-1.Results of recovery test were found in the range from 83% to 96%,and values of lower limit of determination (10S/N) found were 1 μg·kg-1 for dexamethasone and betamethasone,and 2 μg·kg-1 for beclomethasone.