UHPLC Determination of 5 Artificial Edulcorators in Food

UHPLC was applied to the determination of 5 artificial edulcorators, i.e., acesulfame, sodium saccharin, aspartame, dulcin and neotame in food. Methods for pretreatment of different kinds of samples were described in detail. MN NUCLEODUR C18 Pyramid column (3 mm×250 mm, 5.0 μm) was used as stationary phase, and a mixed solution of 5.0 mmol·L-1 acetic acid-ammonium acetate buffer solution of pH 6.0 and methanol in different ratios was used as mobile phase for gradient elution, and PDA detector at the wavelength of 235 nm was used for determination. Linear relationships between values of peak area and mass concentration of the 5 edulcorators were kept in definite ranges. Values of detection limits (3S/N) of the method were found to be 0.476, 0.005, 3.590, 0.002 and 219.9 mg·L-1 respectively. Tests for recovery and precision were made by standard addition method, values of recovery were ranged from 75.0% to 104% and RSD′s (n=5) found were ranged from 0.3% to 7.2%.