Comparison of 2 Derivatization Methods in GC-MS Determination of Residual Butyryl Hydrazide in Peanuts

Methods of derivatization,in GC-MS determination of residual butyryl hydrazide in peanuts,with salicyl aldehyde and with 2-nitrobenzaldehyde as derivatizing reagents were compared with respect to the reaction medium and condition of derivatization,response values,range of linearity of standard curves,and values of lower limit of determination as well as the recovery.It was found that both of the reagents were feasible to be used in the derivatization under the optimum condition,but salicyl aldehyde was preferred due to its easy operation of derivatization reaction,higher sensitivity and better recovery.Removal of the un-reacted salicyl aldehyde by centrifugation was the guarantee for precision of measurements.Lower limit of determination (10S/N) of the method using salicyl aldehyde as derivatizing reagent was found to be 0.005 mg·kg-1 of butyryl hydrazide,and the values of recovery obtained were in the range of 98.0%-99.4%,and the values of RSD′s (n=6) were in the range of 1.5%-3.6%.