HPLC-MS/MS Determination of the Contraband Drug Rimonabant Added in De-obese Health-care Foodstuffs

HPLC-MS/MS was applied to the determination of rimonabant,a contraband drug added in some de-obese health-care foodstuffs.Rimonabant in different kinds of such foodstuffs were separated from the sample and purified by accelerated solvent extraction and solid phase extraction in succession.The eluate was used for HPLC-MS/MS determination,in which the Waters Atlantis T3 column was used as chromatographic column,and a mixture of methanol and 10 mmol·L-1 ammonium acetate solution was used as mobile phase;positive electrospray ionization as well as multiple reaction monitoring mode was used in the detection.Ion pairs used for qualitative analysis were: m/z 462.9,363.0 and m/z 462.9,84.0,ion pair use for quantitative analysis was m/z 462.9,363.0.Linearity range was found between 0.2-500 μg·L-1,and the detection limit (3S/N) found was 0.75 mg·kg-1.Values of recovery found by addition of standard rimonabant,were in the range of 83.7%-101.6%,and values of RSD′s (n=6) of intra-day and inter-day found were less than 8% and 10% respectively.