Rapid Determination of Cholesterol in Oil and Fat

Samples of vegetable or animal oil or fat were treated with absolute ethanol and aliquots of the solutions obtained were used for HPLC determination of cholesterol contents.Analytical column of SB-C18 (4.6 mm×150 mm) was used as the stationary phase and mixture of methanol and water (95+5) was used as the mobile phase at the rate of 1.0 mL·min-1.UV-detections were made at the wavelength of 208 nm.Detection limit (3S/N) of the method was found to be 2.0 mg·L-1.Recovery was tested and the results obtained were in the range of 96.5%-99.0%.