HPLC Determination of Bifenthrin Content in Bifenthrin Suspension

HPLC was applied to the determination of content of bifenthrin in its product of suspension. The sample was extracted with methanol ultrasonically for 20 min. An aliquot of the extract was diluted to 100.0 mL with the mobile phase solution, i.e. a mixture of methanol and water (87+13). A portion of the solution, taking with a filtering injector for purification, was introduced into the instrument and separated on Agilent Eclipse XDB C18 column with the mobile phase solution as eluant at the flow-rate of 1.4 mL·min-1. Diode array detector at the wavelength of 206 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of bifenthrin was kept in the range of 3.0-50.0 mg·L-1, with detection limit (3S/N) 0.67 mg·L-1. Tests for precision and accuracy were carried out at three concentration levels of 11.1, 15.5, 24.4 mg·L-1, giving values of recovery and RSD′s (n=6) in the ranges of 99.2%-104.5% and 0.8%-2.9% respectively.