GC-MS with HS-SPME Determination of Methadone and Its Main Metabolite in Urine

GC-MS with HS-SPME was applied to the determination of contents of methadone and its main metabolite EDDP in urine.Influtential factors to extraction efficiency,including extracting time,extracting temperature,pH value,and ionic strength were studied and optimized.SKF525A was used as internal standard,and the ions with m/z 277,72 and 86 was selected as quantitative ions for the determination of EDDP,methadone and SKF525A,respectively.Linearity ranges for both methadone and EDDP were between 0.01-1.0 mg·L-1,with RSD′s (n=5) less than 9.0%.The values of recovery of methadone and EDDP found by standard addition method were 104.2% and 97.6% respectively.