LC Determination of 3 Components in Faeces Trogopterori with Separation by Accelerated Solvent Extraction

Three components (i.e. rutin, kaempferol and amentoflavone) in faeces trogopterori sample (10 g) was extracted with mixture of C2H5OH-H2O (75+25) thrice for 10 min each in an accelerated solvent extractor under temperature of 100 ℃ and pressure of 15 MPa. The extracts were combined and evaporated to ca. 3 mL;after cooling, its volume was made up to 5.0 mL with the C2H5OH-H2O (75+25) mixture, and the solution was separated on the chromatographic column of Diamosil ODS C18 and eluted gradiently with mixtures of (A) methanol and (B) 0.5% (φ) acetic acid solution with various mixing ratios. UV detection at 360 nm was used in the determination. Linear relationships between values of peak area and mass concentrations of the 3 components were found in definite ranges, with detection limits (3S/N) ranged from 0.3 to 0.6 mg·kg-1. Recovery was tested by standard addition at 3 concentration levels, giving results of recovery in the range of 80.0% to 95.0%, with values of RSD′s (n=5) ranged from 3.2% to 9.6%.