Resonance Rayleigh Scattering of the Reaction System Between Acriflavine Hydrochloride and DNA and Its Analytical Application

Based on the enhancing of resonance rayleigh scattering (RRS) due to the reaction between acriflavine hydrochloride and DNA,a method for the determination of DNA was proposed.Two RRS peaks,a strong peak at 505 nm and a weak peak at 330 nm in a B-R buffer medium of pH 6.4,were observed in the RRS spectrum.Linear relationship between values of RRS intensity and mass concentration of DNA was found in the range of 0.04-0.80 mg·L-1,and value of detection limit (3S/N) found was 0.023 mg·L-1.The proposed method was use in the determination of DNA in 3 simulated samples,giving recovery in the range of 98.0%-104.0%.Mechanism of the reaction was studied,and effects of electrostatic and π-π accumulation were found to be involved in the reaction.