Determination of Residual Amounts of Organochloring Pesticides in Tobacco and in Cigarette Smoke by Capillary Column-Gas Chromatography

Sample of tobacco or of the suspended particles,collected from cigarette smoke using smoking machine and filter,was extracted with n-hexane.The extract was purified by passing through a florisil SPE column.The eluate (in n-hexane) from the column was concentrated to 5 mL and used for GC determination.The DB-5 elastic quartz capillary column was used for separation of the organochlorine pesticides (OCP′s) and ECD was adopted in GC-analysis.Seventeen OCP′s were determined by the method,with values of detection limit (3S/N) ranged from 0.02-0.10 μg·g-1.Recovery and precision were tested by adding standards of the OCP′s at 3 concentration levels to portions of a same tobacco sample and analyzed by the proposed method,values of average recovery and RSD′s (n=7) found were in the ranges of 86%-92% and 3.0%-4.1% respectively.