Color Reaction Among Europium(Ⅲ) Aniline Blue and Amikacin and Its Analytical Application

In a BR buffer solution of pH 4.2,a ternary ion association complex was formed among Eu(Ⅲ),aniline blue and amikacin,with its positive and negative absorption maxima lain at 676 nm and 606 nm respectively,and their molar absorptivity were (ε676) 4.59×104L·mol-1·cm-1 and (ε606) 9.25×104L·mol-1·cm-1.In the mode of dualwavelength measurement,molar absorptivity of 1.38×105L·mol-1·cm-1 was achieved.Linear relationship between the absorbance and concentration of amikacin was obtained in the range of 0-1.5 mol·L-1 (positive absorption) and in the range of 0-1.6 mol·L-1 (negative absorption).Method of spectrophotometric determination of amikacin both in the modes of monowavelength and double wavelength measurement was proposed.In the analysis of samples of amikacin injection and human urine,values of RSD less than 1.5% were obtained.Results of recovery tests found were in the range of 99.7%-100.3%.