Simultaneous Determination of Residual Amounts of Thiabendazole and Carbendazim in Edible Fungus by HPLC with Solid Phase Extraction

Residual amounts of thiabendazole (TBD) and carbendazim (CBD) were extracted ulltrasonically with ethylacetate from samples of edible fungus.The extract was evaporated to dryness by N2-blowing and the residue was dissolved in 0.05 mol·L-1 HCl.TBD and CBD in dil.HCl solution was then purified by SPE.The Waters Symmetry C18 column was used as stationary phase and a mixture of phosphate buffer solution and acetonitrile (mixed in the ratio of 75+25 by volume) was used as mobile phase.UV-detection was made using diode array detector at the wavelength of 288 nm,and external standard were used for quantification.Limit of detection (3S/N) and lower limit of determination (10S/N) of the methd were found to be 0.010 mg·L-1 and 0.025 mg·L-1 respectively for both bactericides.Tests for recovery and precision were made at 4 different concentration levels,and values of recovery found were in the range of 72.0%-86.0% with values of RSD′s (n=6) less than 8.5%.