Analysis of the TCM of Leaves of Microcos Paniculata L. for Its Flavonoides by HPLC Fingerprint Atlas

HPLC fingerprint atlas of flavonoides in the TCM of leaves of Microcos paniculata L. was established by using Kromasil C18 column (250 mm×4.6 mm, 5 μm) as stationary phase and mixed solutions of methanol and dil. phosphoric acid solution (0.1+99.9) in different ratio as mobile phase for gradient elution in the HPLC analysis. Vitexin was used as internal standard and UV-detection at the wavelength of 276 nm was adopted. Samples of the TCM were taken from 9 producing areas in our country and analyzed by the proposed method. HPLC fingerprint atlas showing 18 peaks of flavonoides of the 9 TCM′s was obtained, with good similarities among data of relative retention time and relative peak area of the 9 samples. The established fingerprint was proved to be feasible for use in the quality control of the TCM.