HPLC Determination of Chlorogenic Acid, Baicalin and Puerarin in a TCM Preparation of “Xiao-er Resuqing” Oral Liquor

The effective components, i.e. chlorogenic acid, baicalin and puerarin, in a TCM preparation, named “xiao-er resuqing” oral liquor, were determined by HPLC. The Zorbax SB-C18 chromatographic column was used as the stationary phase and mixtures of methanol and CH3COOH-H2O (1+99) solution with various proportions were used in the gradient elution. UV detections at 327 nm (for chlorogenic aicd and baicalin) and at 250 nm (for puerarin) were adopted in the determinations. Linear relationships between values of peak area and mass concentration of the 3 compounds were found in the following ranges of ① 3.0-30.0 mg·L-1, ② 2.7-27.0 mg·L-1 and ③ 3.1-31.0 mg·L-1 respectively. Values of average recovery obtained were 99.5% (for chlorogenic acid), 99.8% (for baicalin) and 99.3% (for puerarin). Values of RSD′s (n=6) found for the 3 compounds were 0.88%, 0.84% and 1.0% respectively.